Generator

Part:BBa_K843001:Design

Designed by: Berke Gürkan   Group: iGEM12_METU   (2012-09-26)


Carbon Monoxide Dehydrogenase generator with constitutive promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1331
    Illegal BamHI site found at 394
    Illegal BamHI site found at 1290
    Illegal BamHI site found at 1848
    Illegal BamHI site found at 2634
    Illegal XhoI site found at 3175
    Illegal XhoI site found at 3595
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 116
    Illegal NgoMIV site found at 774
    Illegal NgoMIV site found at 901
    Illegal NgoMIV site found at 2439
    Illegal NgoMIV site found at 3283
    Illegal NgoMIV site found at 3696
    Illegal AgeI site found at 1793
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1945
    Illegal BsaI.rc site found at 2728
    Illegal BsaI.rc site found at 3613


Design Notes

We changed a base in the CODH coding region since it contained XbaI cut site.


Source

Medium chain : http://www.ncbi.nlm.nih.gov/gene/11808648

Small chain: http://www.ncbi.nlm.nih.gov/gene/11808647

Large chain: http://www.ncbi.nlm.nih.gov/gene/11808646

https://parts.igem.org/wiki/index.php?title=Part:BBa_J23116

References